Fluorescence Compensation webinar

“Advanced 4-18 Color Compensation Strategies For 2019” – Free webinar from Excyte

The webinar starts Thursday, October 24th at 1 PM EDT.

One of the most common mistakes that has been passed down incorrectly by word of mouth over the years is the idea that it’s “okay” to do manual compensation.

Manual compensation is not okay.

Manual compensation is the process of adjusting the compensation based on how the data visually looks.

If you have manually compensated data in your lab notebook – strike it out, and then redo your experiment.

Why?

Because manual compensation results in overcompensated data, yielding incorrect conclusions.

The best practice is to use automatic compensation algorithms that are available in the most current versions of flow cytometry software.

For automatic compensation to be successful, the following 3 rules must be followed:

• Controls must be at least as bright as the samples they will be applied to. Brighter is better, but not off scale.

• Background fluorescence should be the same between the negative and positive population. Avoid using the universal negative for compensation.

• The compensation color must be the same as the experimental color. For example, don’t use Alexa488 to compensate for FITC.

Following these three rules is easy when you’re doing a 1-color, 2-color, or even a 3-color flow cytometry experiment.

However, the process of compensating your experiment properly becomes much more complicated when you’re preparing 4-18 color experiments.

For this reason, we’ve put together a rare, FREE public webinar that will show you how to compensate 4-18 color experiments