LOCUS       pOME0092       440 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  MERS CoV England ORF 8b MUTANT NT 119 T -->C tagged nt 287129-29067; vector pDEST47
SOURCE      MERS
  ORGANISM  MERS
            MERS CoV England ORF 8b Mutant tagged; UNC163-18; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            Plasmid received from RG
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..423
                     /cds_type=ORF
                     /note="Length: 405"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            355..420
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            421..426
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(427..432,427^428)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 427^428"
                     /note="cut_0_on_negative_strand: 431^432"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            427..440
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(433..440,434^435)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 434^435"
                     /note="cut_0_on_negative_strand: 438^439"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GCCAATTCCA CCCCTGCGCA AAATGCTGGG TATTGGCGGA
       61 GACAGGACAG AAAAATTAAT ACCGGGAATG GAATTAAGCA ACTGGCTCCC AGGTGGTACT
      121 TCTACTACAC TGGAACTGGA CCCGAAGCAG CACTCCCATT CCGGGCTGTT AAGGATGGCA
      181 TCGTTTGGGT CCATGAAGAT GGCGCCACTG ATGCTCCTTC AACTTTTGGG ACGCGGAACC
      241 CTAACAATGA TTCAGCTATT GTTACACAAT TCGCGCCCGG TACTAAGCTT CCTAAAAACT
      301 TCCACATTGA GGGGACTGGA GGCAATAGTC AATCATCTTC AAGAGCCTCT AGCGgactac
      361 aaagaccatg acggtgatta taaagatcat gatatcgatt acaaggatga cgatgacaag
      421 tagtaactcg aggcggccgc
//