LOCUS       pOME0178      2286 bp    DNA     linear   UNA 29-Jan-2014
DEFINITION  KSHV ORF22 TAGGED; vector pDEST47
SOURCE      UNC166-8 (KSHV hyp ORF22 TAGGED)
COMMENT     5' end RE changed to XmnI to avoid internal sites of SalI and KpnI
            3' end only cut with NotI to avoid XhoI internal site
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            
FEATURES             Location/Qualifiers
     Site            join(1..10,5^6)
                     /site_type="restriction site"
                     /note="Name: XmnI"
                     /note="Pattern: gaannnnttc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XmnI
     Site            1..10
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            11..16
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             17..2275
                     /cds_type=ORF
                     /note="Length: 2259"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            2207..2272
                     /site_type="mutagenized site"
                     /label="3X FLAG"
     Site            2273..2278
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(2279..2286,2280^2281)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 2280^2281"
                     /note="cut_0_on_negative_strand: 2284^2285"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            2279..2286
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gaaccaattc gccaccatgc agggtctagc cttcttggcg gcccttgcat gctggcgatg
       61 catatcgttg acatgtggag ccactggcgc gttgccgaca acggcgacga caataacccg
      121 ctccgccacg cagctcatca atgggagaac caacctctcc atagaactgg aattcaacgg
      181 cactagtttt tttctaaatt ggcaaaatct gttgaatgtg atcacggagc cggccctgac
      241 agagttgtgg acctccgccg aagtcgccga ggacctcagg gtaactctga aaaagaggca
      301 aagtcttttt ttccccaaca agacagttgt gatctctgga gacggccatc gctatacgtg
      361 cgaggtgccg acgtcgtcgc aaacttataa catcaccaag ggctttaact atagcgctct
      421 gcccgggcac cttggcggat ttgggatcaa cgcgcgtctg gtactgggtg atatcttcgc
      481 atcaaaatgg tcgctattcg cgagggacac cccagagtat cgggtgtttt acccaatgaa
      541 tgtcatggcc gtcaagtttt ccatatccat tggcaacaac gagtccggcg tagcgctcta
      601 tggagtggtg tcggaagatt tcgtggtcgt cacgctccac aacaggtcca aagaggctaa
      661 cgagacggcg tcccatcttc tgttcggtct cccggattca ctgccatctc tgaagggcca
      721 tgccacctat gatgaactca cgttcgcccg aaacgcaaaa tatgcgctag tggcgatcct
      781 gcctaaagat tcttaccaga cactccttac agagaattac actcgcatat ttctgaacat
      841 gacggagtcg acgcccctcg agttcacgcg gacgatccag accaggatcg tatcaatcga
      901 ggccaggcgc gcctgcgcag ctcaagaggc ggcgccggac atattcttgg tgttgtttca
      961 gatgttggtg gcacactttc ttgttgcgcg gggcattgcc gagcaccgat ttgtggaggt
     1021 ggactgcgtg tgtcggcagt atgcggaact gtattttctc cgccgcatct cgcgtctgtg
     1081 catgcccacg ttcaccactg tcgggtataa ccacaccacc cttggcgctg tggccgccac
     1141 acaaatagct cgcgtgtccg ccacgaagtt ggccagtttg ccccgctctt cccaggaaac
     1201 agtgctggcc atggtccagc ttggcgcccg tgatggcgcc gtcccttcct ccattctgga
     1261 gggcattgct atggtcgtcg aacatatgta taccgcctac acttatgtgt acacactcgg
     1321 cgatactgaa agaaaattaa tgttggacat acacacggtc ctcaccgaca gctgcccgcc
     1381 caaagactcc ggagtatcag aaaagctact gagaacatat ttgatgttca catcaatgtg
     1441 taccaacata gagctgggcg aaatgatcgc ccgcttttcc aaaccggaca gccttaacat
     1501 ctatagggca ttctccccct gctttctagg actaaggtac gatttgcatc cagccaagtt
     1561 gcgcgccgag gcgccgcagt cgtccgctct gacgcggact gccgttgcca gaggaacatc
     1621 gggattcgca gaattgctcc acgcgctgca cctcgatagc ttaaatttaa ttccggcgat
     1681 taactgttca aagattacag ccgacaagat aatagctacg gtacccttgc ctcacgtcac
     1741 gtatatcatc agttccgaag cactctcgaa cgctgttgtc tacgaggtgt cggagatctt
     1801 cctcaagagt gccatgttta tatctgctat caaacccgat tgctccggct ttaacttttc
     1861 tcagattgat aggcacattc ccatagtcta caacatcagc acaccaagaa gaggttgccc
     1921 cctttgtgac tctgtaatca tgagctacga tgagagcgat ggcctgcagt ctctcatgta
     1981 tgtcactaat gaaagggtgc agaccaacct ctttttagat aagtcacctt tctttgataa
     2041 taacaaccta cacattcatt atttgtggct gagggacaac gggaccgtag tggagataag
     2101 gggcatgtat agaagacgcg cagccagtgc tttgtttcta attctctctt ttattgggtt
     2161 ctcgggggtt atctactttc tttacagact gttttccatc ctttatgact acaaagacca
     2221 tgacggtgat tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaagc
     2281 ggccgc
//