LOCUS       pOME0116      2627 bp    DNA     linear   UNA 26-Nov-2013
DEFINITION  KSHV ORF 8 gB GQ994935 TAGGED
COMMENT     Internal SalI and XhoI sites
FEATURES             Location/Qualifiers
     Site            1..6
                     /site_type="restriction site"
                     /label=KpnI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..2616
                     /cds_type=ORF
                     /note="Length: 2604"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            2548..2613
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            2614..2619
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            2620..2627
                     /site_type="restriction site"
                     /label=NotI
     Site            2620..2627
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 ggtaccgcca ccatgactcc caggtctaga ttggccaccc tggggactgt catcctgttg
       61 gtctgctttt gcgcaggcgc ggcgcactcg aggggtgaca cctttcagac gtccagttcc
      121 cccacacccc caggatcttc ctctaaggcc cccaccaaac ctggtgagga agcatctggt
      181 cctaagagtg tggactttta ccagttcaga gtgtgtagtg catcgatcac cggggagctt
      241 tttcggttca acctggagca gacgtgccca gacaccaaag acaagtacca ccaagaagga
      301 attttactgg tgtacaaaaa aaacatagtg cctcatatct ttaaggtgcg gcgctatagg
      361 aaaattgcca cctctgtcac ggtctacagg ggcttgacag agtccgccat caccaacaag
      421 tatgaactcc cgagacccgt gccactctat gagataagcc acatggacag cacctatcag
      481 tgctttagtt ccatgaaggt aaatgtcaac ggggtagaaa acacatttac tgacagagac
      541 gatgttaaca ccacagtatt cctccaacca gtagaggggc ttacggataa cattcaaagg
      601 tactttagcc agccggtcat ctacgcggaa cccggctggt ttcccggcat atacagagtt
      661 aggaccaccg tcaattgcga gatagtggac atgatagcca ggtctgctga accatacaat
      721 tactttgtca cgtcactggg tgacacggtg gaagtctccc ctttttgcta taacgaatcc
      781 tcatgcagca caacccccag caacaaaaat ggccttagcg tccaagtagt tctcaaccac
      841 actgtggtca cgtactctga cagaggaacc agtcccactc cccaaaacag gatctttgtg
      901 gaaacgggag cgtacacgct ttcgtgggcc tccgagagca agaccacggc cgtgtgtccg
      961 ctggcactgt ggaaaacctt cccgcgctcc atccagacta cccacgagga cagcttccac
     1021 tttgtggcca acgagatcac ggccaccttc acggctcctc taacgccagt ggccaacttt
     1081 accgacacgt actcttgtct gacctcggat atcaacacca cgctaaacgc cagcaaggcc
     1141 aaactggcga gcactcacgt ccctaacggg acggtccagt acttccacac aacaggcgga
     1201 ctctatttgg tctggcagcc catgtccgcg attaacctga ctcacgctca gggcgacagc
     1261 gggaacccca cgtcatcgcc gcccccctcc gcatccccca tgaccacctc tgccagccgc
     1321 agaaagagac ggtcagccag taccgctgct gccggcggcg gggggtccac ggacaacctg
     1381 tcttacacgc agctgcagtt tgcctacgac aaactgcggg atggcattaa tcaggtgtta
     1441 gaagaactct ccagggcatg gtgtcgcgag caggtcaggg acaacctaat gtggtacgag
     1501 ctcagtaaaa tcaaccccac cagcgttatg acagccatct acggtcgacc tgtatccgcc
     1561 aagttcgtag gagacgccat ttccgtgacc gagtgcatta acgtggacca gagctccgta
     1621 aacatccaca agagcctcag aaccaatagt aaggacgtgt gttacgcgcg ccccctggtg
     1681 acgtttaagt ttttgaacag ttccaaccta ttcaccggcc agctgggcgc gcgcaatgag
     1741 ataatactga ccaacaacca ggtggaaacc tgcaaagaca cctgcgaaca ctacttcatc
     1801 acccgcaacg agactctggt gtataaggac tacgcgtacc tgcgcactat aaacaccact
     1861 gacatatcca ccctgaacac ttttatcgcc ctgaatctat cctttattca aaacatagac
     1921 ttcaaggcca tcgagctgta cagcagtgca gagaaacgac tcgcgagtag cgtgtttgac
     1981 ctggagacga tgttcaggga gtacaactac tacacacatc gtctcgcggg tttgcgcgag
     2041 gatctggaca acaccataga tatgaacaag gagcgcttcg taagggactt gtcggagata
     2101 gtggcggacc tgggtggcat cggaaaaacg gtggtgaacg tggccagcag cgtggtcact
     2161 ctatgtggct cattggttac cggattcata aattttatta aacaccccct aggtggcatg
     2221 ctgatgatca ttatcgttat agcaatcatc ctgatcattt ttatgctcag tcgccgcacc
     2281 aataccatag cccaggcgcc ggtgaagatg atctaccccg acgtagatcg cagggcacct
     2341 cctagcggcg gagccccaac acgggaggaa atcaaaaaca tcctgctggg aatgcaccag
     2401 ctacaacaag aggagaggca gaaggcggat gatatgaaaa aaagtacacc ctcggtgttt
     2461 cagcgtaccg caaacggcct tcgtcagcgt ctgagaggat ataaacctct gactcaatcg
     2521 ctagacatca gtccggaaac gggggaggac tacaaagacc atgacggtga ttataaagat
     2581 catgatatcg attacaagga tgacgatgac aagtagtaag cggccgc
//